Journal: Biologics : Targets & Therapy

Article Title: Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions

doi: 10.2147/BTT.S22863

Figure Lengend Snippet: Globular adiponectin is localized in the fibrous cap of atherosclerotic plaques. ( A ) For double-staining experiments, aortic sections of apoprotein E-deficient mice were coincubated with globular adiponectin-Atto655 20 μg/mL and AlexaFluor488-labeled rat antimouse CD31 antibody. The nuclei were stained with Hoechst (blue). Single Z-plane fluorescence images were taken at a depth of 4 μm from the top of an atherosclerotic plaque showing the localization of globular adiponectin-Atto655 in the endothelial layer. The aortic surface is shown in the small inserts. ( B ) Aortic sections of apoprotein E-deficient mice were coincubated with globular adiponectin-Atto655 and AlexaFluor488-labeled rabbit antihuman collagen type I antibody. Single Z-plane fluorescence images were taken at a depth of 7 μm from the top of an atherosclerotic plaque, showing colocalization of globular adiponectin and collagen type I. ( C ) For negative control experiments, aortic sections were preincubated with a high amount of native globular adiponectin. A stack of optical sections from an atherosclerotic plaque projected into one plane showed only a weak globular adiponectin-Atto655 signal, compared with the plaque image in the left panel in B . ( D ) Single Z-plane fluorescence light images from a globular adiponectin-Atto655 and an interleukin 10-Atto655-stained plaque at a depth of 10 μm from top of the plaque. Images show the different staining patterns between globular adiponectin and interleukin 10. Notes: Scale bars: A and C , 50 μm; B , 20 μm.

Article Snippet: A rabbit antihuman collagen type I antibody (Rockland Immunochemicals, Philadelphia, PA) was used as a positive control, and nonspecific rat antihuman IgG2a as a negative control, similar in molecular weight (150 kDa) to the fAd-Sf hexamer (167 kDa).

Techniques: Double Staining, Labeling, Staining, Fluorescence, Negative Control

Journal: Advanced healthcare materials

Article Title: Conditioning of 3D Printed Nanoengineered Ionic-Covalent Entanglement Scaffolds with iP-hMSCs Derived Matrix

doi: 10.1002/adhm.201901580

Figure Lengend Snippet: Primers used in this study.

Article Snippet: Scaffolds were then incubated overnight at 4°C in either rabbit antihuman type VI collagen (Novus Biologicals, Littleton, CO) or rabbit antihuman type XII collagen antibody (Novus) diluted in the blocking buffer at a 1:200 ratio.

Techniques: Sequencing